Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRF5 / GPR116 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113376-100
Catalog Number: LS-G113376
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR116(ADGRF5) (NM_015234), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Ig-Hepta homolog | Ig-hepta | G protein-coupled receptor 116 | GPR116 | KIAA0758 | KPG_001
- Origin Species
- Human
- NCBI GenBank
- NM_015234
- SwissProt
- Q8IZF2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 149.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKSPRRTTLC LMFIVIYSSK AALNWNYEST IHPLSLHEHE PAGEEALRQK RAVATKSPTA61EEYTVNIEIS FENASFLDPI KAYLNSLSFP IHGNNTDQIT DILSINVTTV CRPAGNEIWC121SCETGYGWPR ERCLHNLICQ ERDVFLPGHH CSCLKELPPN GPFCLLQEDV TLNMRVRLNV181GFQEDLMNTS SALYRSYKTD LETAFRKGYG ILPGFKGVTV TGFKSGSVVV TYEVKTTPPS241LELIHKANEQ VVQSLNQTYK MDYNSFQAVT INESNFFVTP EIIFEGDTVS LVCEKEVLSS301NVSWRYEEQQ LEIQNSSRFS IYTALFNNMT SVSKLTIHNI TPGDAGEYVC KLILDIFEYE361CKKKIDVMPI QILANEEMKV MCDNNPVSLN CCSQGNVNWS KVEWKQEGKI NIPGTPETDI421DSSCSRYTLK ADGTQCPSGS SGTTVIYTCE FISAYGARGS ANIKVTFISV ANLTITPDPI481SVSEGQNFSI KCISDVSNYD EVYWNTSAGI KIYQRFYTTR RYLDGAESVL TVKTSTREWN541GTYHCIFRYK NSYSIATKDV IVHPLPLKLN IMVDPLEATV SCSGSHHIKC CIEEDGDYKV601TFHTGSSSLP AAKEVNKKQV CYKHNFNASS VSWCSKTVDV CCHFTNAANN SVWSPSMKLN661LVPGENITCQ DPVIGVGEPG KVIQKLCRFS NVPSSPESPI GGTITYKCVG SQWEEKRNDC721ISAPINSLLQ MAKALIKSPS QDEMLPTYLK DLSISIDKAE HEISSSPGSL GAIINILDLL781STVPTQVNSE MMTHVLSTVN VILGKPVLNT WKVLQQQWTN QSSQLLHSVE RFSQALQSGD841SPPLSFSQTN VQMSSMVIKS SHPETYQQRF VFPYFDLWGN VVIDKSYLEN LQSDSSIVTM901AFPTLQAILA QDIQENNFAE SLVMTTTVSH NTTMPFRISM TFKNNSPSGG ETKCVFWNFR961LANNTGGWDS SGCYVEEGDG DNVTCICDHL TSFSILMSPD SPDPSSLLGI LLDIISYVGV1021GFSILSLAAC LVVEAVVWKS VTKNRTSYMR HTCIVNIAAS LLVANTWFIV VAAIQDNRYI1081LCKTACVAAT FFIHFFYLSV FFWMLTLGLM LFYRLVFILH ETSRSTQKAI AFCLGYGCPL1141AISVITLGAT QPREVYTRKN VCWLNWEDTK ALLAFAIPAL IIVVVNITIT IVVITKILRP1201SIGDKPCKQE KSSLFQISKS IGVLTPLLGL TWGFGLTTVF PGTNLVFHII FAILNVFQGL1261FILLFGCLWD LKVQEALLNK FSLSRWSSQH SKSTSLGSST PVFSMSSPIS RRFNNLFGKT1321GTYNVSTPEA TSSSLENSSS ASSLLNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1346 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.