Immunohistochemical analysis of GPR116 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

IHC-plus™ ADGRF5 / GPR116 Antibody (N-Terminus)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human
Format:	Liquid

$383.00each

Catalog Number: LS-B15709-50

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Overview

Description: GPR116 antibody LS-B15709 is an unconjugated rabbit polyclonal antibody to human GPR116 (ADGRF5) (N-Terminus). Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application: ICC, IF, IHC, IHC-P, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Ig-Hepta homolog | Ig-hepta | G protein-coupled receptor 116 | GPR116 | KIAA0758 | KPG_001
Recommended Dilution: IF/ICC: 1:100 - 1:500
Recommended Dilution: IHC-P: 5 µg/ml
Recommended Dilution: WB: 1:500 - 1:1000
UniProt Number: Q8IZF2
NCBI GenBank: AAN46672.1 | NM_015234
SwissProt: Q8IZF2

Target

Target: ADGRF5 / GPR116
Antigen Species: Human
Specificity: Recognizes endogenous levels of GPR116 protein.
Epitope: Recognizes endogenous levels of GPR116 protein.
Gene Name: ADGRF5

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human GPR116.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.42% Potassium Phosphate, pH 7.3, 0.87% NaCl, 0.01% Sodium Azide, 30% Glycerol
Concentration: 1 mg/mL
Volume: 50 Microliter
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Affinity Purified