Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRE3 / EMR3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108720-100
Catalog Number: LS-G108720
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EMR3(ADGRE3) (NM_032571), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EGF-like module receptor 3 | EMR3
- Origin Species
- Human
- NCBI GenBank
- NM_032571
- SwissProt
- Q9BY15
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQGPLLLPGL CFLLSLFGAV TQKTKTSCAK CPPNASCVNN THCTCNHGYT SGSGQKLFTF61PLETCNDINE CTPPYSVYCG FNAVCYNVEG SFYCQCVPGY RLHSGNEQFS NSNENTCQDT121TSSKTTEGRK ELQKIVDKFE SLLTNQTLWR TEGRQEISST ATTILRDVES KVLETALKDP181EQKVLKIQND SVAIETQAIT DNCSEERKTF NLNVQMNSMD IRCSDIIQGD TQGPSAIAFI241SYSSLGNIIN ATFFEEMDKK DQVYLNSQVV SAAIGPKRNV SLSKSVTLTF QHVKMTPSTK301KVFCVYWKST GQGSQWSRDG CFLIHVNKSH TMCNCSHLSS FAVLMALTSQ EEDPVLTVIT361YVGLSVSLLC LLLAALTFLL CKAIQNTSTS LHLQLSLCLF LAHLLFLVGI DRTEPKVLCS421IIAGALHYLY LAAFTWMLLE GVHLFLTARN LTVVNYSSIN RLMKWIMFPV GYGVPAVTVA481ISAASWPHLY GTADRCWLHL DQGFMWSFLG PVCAIFSANL VLFILVFWIL KRKLSSLNSE541VSTIQNTRML AFKATAQLFI LGCTWCLGLL QVGPAAQVMA YLFTIINSLQ GFFIFLVYCL601LSQQVQKQYQ KWFREIVKSK SESETYTLSS KMGPDSKPSE GDVFPGQVKR KYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
652 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.