Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADCY1 / Adenylate Cyclase 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110764-100
Catalog Number: LS-G110764
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Adenylate cyclase 1(ADCY1) (NM_021116), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 3 | 5-cyclic AMP synthetase | Adenylate cyclase type I | AC1 | Adenylate cyclase type 1 | Adenylyl cyclase 1 | ATP pyrophosphate-lyase 1 | ADCY1 | Adenyl cyclase | Adenylate cyclase 1 (brain) | Adenylate cyclase | type i | ATP pyrophosphate-lyase
- Origin Species
- Human
- NCBI GenBank
- L05500
- SwissProt
- Q08828
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 123.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGAPRGGGG GGGGAGEPGG AERAAGTSRR RGLRACDEEF ACPELEALFR GYTLRLEQAA61TLKALAVLSL LAGALALAEL LGAPGPAPGL AKGSHPVHCV LFLALLVVTN VRSLQVPQLQ121QVGQLALLFS LTFALLCCPF ALGGPARGSA GAAGGPATAE QGVWQLLLVT FVSYALLPVR181SLLAIGFGLV VAASHLLVTA TLVPAKRPRL WRTLGANALL FVGVNMYGVF VRILTERSQR241KAFLQARSCI EDRLRLEDEN EKQERLLMSL LPRNVAMEMK EDFLKPPERI FHKIYIQRHD301NVSILFADIV GFTGLASQCT AQELVKLLNE LFGKFDELAT ENHCRRIKIL GDCYYCVSGL361TQPKTDHAHC CVEMGLDMID TITSVAEATE VDLNMRVGLH TGRVLCGVLG LRKWQYDVWS421NDVTLANVME AAGLPGKVHI TKTTLACLNG DYEVEPGYGH ERNSFLKTHN IETFFIVPSH481RRKIFPGLIL SDIKPAKRMK FKTVCYLLVQ LMHCRKMFKA EIPFSNVMTC EDDDKRRALR541TASEKLRNRS SFSTNVVYTT PGTRVNRYIS RLLEARQTEL EMADLNFFTL KYKHVEREQK601YHQLQDEYFT SAVVLTLILA ALFGLVYLLI FPQSVVVLLL LVFCICFLVA CVLYLHITRV661QCFPGCLTIQ IRTVLCIFIV VLIYSVAQGC VVGCLPWAWS SKPNSSLVVL SSGGQRTALP721TLPCESTHHA LLCCLVGTLP LAIFFRVSSL PKMILLSGLT TSYILVLELS GYTRTGGGAV781SGRSYEPIVA ILLFSCALAL HARQVDIRLR LDYLWAAQAE EEREDMEKVK LDNRRILFNL841LPAHVAQHFL MSNPRNMDLY YQSYSQVGVM FASIPNFNDF YIELDGNNMG VECLRLLNEI901IADFDELMEK DFYKDIEKIK TIGSTYMAAV GLAPTSGTKA KKSISSHLST LADFAIEMFD961VLDEINYQSY NDFVLRVGIN VGPVVAGVIG ARRPQYDIWG NTVNVASRMD STGVQGRIQV1021TEEVHRLLRR CPYHFVCRGK VSVKGKGEML TYFLEGRTDG NGSQIRSLGL DRKMCPFGRA1081GLQGRRPPVC PMPGVSVRAG LPPHSPGQYL PSAAAGKEATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1119 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.