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Human ADCY1 / Adenylate Cyclase 1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ADCY1 / Adenylate Cyclase 1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ADCY1 antibodies →
$220.00each
Catalog Number: LS-G74326-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Adenylate cyclase 1(ADCY1) (NM_021116) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
3 | 5-cyclic AMP synthetase | Adenylate cyclase type I | AC1 | Adenylate cyclase type 1 | Adenylyl cyclase 1 | ATP pyrophosphate-lyase 1 | ADCY1 | Adenyl cyclase | Adenylate cyclase 1 (brain) | Adenylate cyclase | type i | ATP pyrophosphate-lyase
Origin Species
Human
NCBI GenBank
L05500
SwissProt
Q08828

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
123.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGAPRGGGG GGGGAGEPGG AERAAGTSRR RGLRACDEEF ACPELEALFR GYTLRLEQAA
61TLKALAVLSL LAGALALAEL LGAPGPAPGL AKGSHPVHCV LFLALLVVTN VRSLQVPQLQ
121QVGQLALLFS LTFALLCCPF ALGGPARGSA GAAGGPATAE QGVWQLLLVT FVSYALLPVR
181SLLAIGFGLV VAASHLLVTA TLVPAKRPRL WRTLGANALL FVGVNMYGVF VRILTERSQR
241KAFLQARSCI EDRLRLEDEN EKQERLLMSL LPRNVAMEMK EDFLKPPERI FHKIYIQRHD
301NVSILFADIV GFTGLASQCT AQELVKLLNE LFGKFDELAT ENHCRRIKIL GDCYYCVSGL
361TQPKTDHAHC CVEMGLDMID TITSVAEATE VDLNMRVGLH TGRVLCGVLG LRKWQYDVWS
421NDVTLANVME AAGLPGKVHI TKTTLACLNG DYEVEPGYGH ERNSFLKTHN IETFFIVPSH
481RRKIFPGLIL SDIKPAKRMK FKTVCYLLVQ LMHCRKMFKA EIPFSNVMTC EDDDKRRALR
541TASEKLRNRS SFSTNVVYTT PGTRVNRYIS RLLEARQTEL EMADLNFFTL KYKHVEREQK
601YHQLQDEYFT SAVVLTLILA ALFGLVYLLI FPQSVVVLLL LVFCICFLVA CVLYLHITRV
661QCFPGCLTIQ IRTVLCIFIV VLIYSVAQGC VVGCLPWAWS SKPNSSLVVL SSGGQRTALP
721TLPCESTHHA LLCCLVGTLP LAIFFRVSSL PKMILLSGLT TSYILVLELS GYTRTGGGAV
781SGRSYEPIVA ILLFSCALAL HARQVDIRLR LDYLWAAQAE EEREDMEKVK LDNRRILFNL
841LPAHVAQHFL MSNPRNMDLY YQSYSQVGVM FASIPNFNDF YIELDGNNMG VECLRLLNEI
901IADFDELMEK DFYKDIEKIK TIGSTYMAAV GLAPTSGTKA KKSISSHLST LADFAIEMFD
961VLDEINYQSY NDFVLRVGIN VGPVVAGVIG ARRPQYDIWG NTVNVASRMD STGVQGRIQV
1021TEEVHRLLRR CPYHFVCRGK VSVKGKGEML TYFLEGRTDG NGSQIRSLGL DRKMCPFGRA
1081GLQGRRPPVC PMPGVSVRAG LPPHSPGQYL PSAAAGKEA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1119 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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