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ABCC10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ABCC10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ABCC10 antibodies →
$725.00each
Catalog Number: LS-G131202-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ABCC10 (NM_001198934), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ABCC10 | EST182763 | Multidrug resistance protein 7 | MRP7 | SIMRP7
Origin Species
Human
NCBI GenBank
NM_033450
SwissProt
Q5T3U5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
162.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MERLLAQLCG SSAAWPLPLW EGDTTGHCFT QLVLSALPHA LLAVLSACYL GTPRSPDYIL
61PCSPGWRLRL AASFLLSVFP LLDLLPVALP PGAGPGPIGL EVLAGCVAAV AWISHSLALW
121VLAHSPHGHS RGPLALALVA LLPAPALVLT VLWHCQRGTL LPPLLPGPMA RLCLLILQLA
181ALLAYALGWA APGGPREPWA QEPLLPEDQE PEVAEDGESW LSRFSYAWLA PLLARGACGE
241LRQPQDICRL PHRLQPTYLA RVFQAHWQEG ARLWRALYGA FGRCYLALGL LKLVGTMLGF
301SGPLLLSLLV GFLEEGQEPL SHGLLYALGL AGGAVLGAVL QNQYGYEVYK VTLQARGAVL
361NILYCKALQL GPSRPPTGEA LNLLGTDSER LLNFAGSFHE AWGLPLQLAI TLYLLYQQVG
421VAFVGGLILA LLLVPVNKVI ATRIMASNQE MLQHKDARVK LVTELLSGIR VIKFCGWEQA
481LGARVEACRA RELGRLRVIK YLDAACVYLW AALPVVISIV IFITYVLMGH QLTATKVFTA
541LALVRMLILP LNNFPWVING LLEAKVSLDR IQLFLDLPNH NPQAYYSPDP PAEPSTVLEL
601HGALFSWDPV GTSLETFISH LEVKKGMLVG IVGKVGCGKS SLLAAIAGEL HRLRGHVAVR
661GLSKGFGLAT QEPWIQFATI RDNILFGKTF DAQLYKEVLE ACALNDDLSI LPAGDQTEVG
721EKGVTLSGGQ RARIALARAV YQEKELYLLD DPLAAVDADV ANHLLHRCIL GMLSYTTRLL
781CTHRTEYLER ADAVLLMEAG RLIRAGPPSE ILPLVQAVPK AWAENGQESD SATAQSVQNP
841EKTKEGLEEE QSTSGRLLQE ESKKEGAVAL HVYQAYWKAV GQGLALAILF SLLLMQATRN
901AADWWLSHWI SQLKAENSSQ EAQPSTSPAS MGLFSPQLLL FSPGNLYIPV FPLPKAAPNG
961SSDIRFYLTV YATIAGVNSL CTLLRAVLFA AGTLQAAATL HRRLLHRVLM APVTFFNATP
1021TGRILNRFSS DVACADDSLP FILNILLANA AGLLGLLAVL GSGLPWLLLL LPPLSIMYYH
1081VQRHYRASSR ELRRLGSLTL SPLYSHLADT LAGLSVLRAT GATYRFEEEN LRLLELNQRC
1141QFATSATMQW LDIRLQLMGA AVVSAIAGIA LVQHQQGLAN PGLVGLSLSY ALSLTGLLSG
1201LVSSFTQTEA MLVSVERLEE YTCDLPQEPQ GQPLQLGTGW LTQGGVEFQD VVLAYRPGLP
1261NALDGVTFCV QPGEKLGIVG RTGSGKSSLL LVLFRLLEPS SGRVLLDGVD TSQLELAQLR
1321SQLAIIPQEP FLFSGTVREN LDPQGLHKDR ALWQALKQCH LSEVITSMGG LDGELGEGGR
1381SLSLGQRQLL CLARALLTDA KILCIDEATA SVDQKTDQLL QQTICKRFAN KTVLTIAHRL
1441NTILNSDRVL VLQAGRVVEL DSPATLRNQP HSLFQQLLQS SQQGVPASLG GP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1492 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.