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ABCC10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ABCC10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ABCC10 antibodies →
$725.00each
Catalog Number: LS-G108215-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ABCC10 (NM_033450), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ABCC10 | EST182763 | Multidrug resistance protein 7 | MRP7 | SIMRP7
Origin Species
Human
NCBI GenBank
NM_033450
SwissProt
Q5T3U5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
158.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCLLVFPLVP RSPDYILPCS PGWRLRLAAS FLLSVFPLLD LLPVALPPGA GPGPIGLEVL
61AGCVAAVAWI SHSLALWVLA HSPHGHSRGP LALALVALLP APALVLTVLW HCQRGTLLPP
121LLPGPMARLC LLILQLAALL AYALGWAAPG GPREPWAQEP LLPEDQEPEV AEDGESWLSR
181FSYAWLAPLL ARGACGELRQ PQDICRLPHR LQPTYLARVF QAHWQEGARL WRALYGAFGR
241CYLALGLLKL VGTMLGFSGP LLLSLLVGFL EEGQEPLSHG LLYALGLAGG AVLGAVLQNQ
301YGYEVYKVTL QARGAVLNIL YCKALQLGPS RPPTGEALNL LGTDSERLLN FAGSFHEAWG
361LPLQLAITLY LLYQQVGVAF VGGLILALLL VPVNKVIATR IMASNQEMLQ HKDARVKLVT
421ELLSGIRVIK FCGWEQALGA RVEACRAREL GRLRVIKYLD AACVYLWAAL PVVISIVIFI
481TYVLMGHQLT ATKVFTALAL VRMLILPLNN FPWVINGLLE AKVSLDRIQL FLDLPNHNPQ
541AYYSPDCGRL GAQIKWLLCS DPPAEPSTVL ELHGALFSWD PVGTSLETFI SHLEVKKGML
601VGIVGKVGCG KSSLLAAIAG ELHRLRGHVA VRGLSKGFGL ATQEPWIQFA TIRDNILFGK
661TFDAQLYKEV LEACALNDDL SILPAGDQTE VGEKGVTLSG GQRARIALAR AVYQEKELYL
721LDDPLAAVDA DVANHLLHRC ILGMLSYTTR LLCTHRTEYL ERADAVLLME AGRLIRAGPP
781SEILPLVQAV PKAWAENGQE SDSATAQSVQ NPEKTKEGLE EEQSTSGRLL QEESKKEGAV
841ALHVYQAYWK AVGQGLALAI LFSLLLMQAT RNAADWWLSH WISQLKAENS SQEAQPSTSP
901ASMGLFSPQL LLFSPGNLYI PVFPLPKAAP NGSSDIRFYL TVYATIAGVN SLCTLLRAVL
961FAAGTLQAAA TLHRRLLHRV LMAPVTFFNA TPTGRILNRF SSDVACADDS LPFILNILLA
1021NAAGLLGLLA VLGSGLPWLL LLLPPLSIMY YHVQRHYRAS SRELRRLGSL TLSPLYSHLA
1081DTLAGLSVLR ATGATYRFEE ENLRLLELNQ RCQFATSATM QWLDIRLQLM GAAVVSAIAG
1141IALVQHQQGL ANPGLVGLSL SYALSLTGLL SGLVSSFTQT EAMLVSVERL EEYTCDLPQE
1201PQGQPLQLGT GWLTQGGVEF QDVVLAYRPG LPNALDGVTF CVQPGEKLGI VGRTGSGKSS
1261LLLVLFRLLE PSSGRVLLDG VDTSQLELAQ LRSQLAIIPQ EPFLFSGTVR ENLDPQGLHK
1321DRALWQALKQ CHLSEVITSM GGLDGELGEG GRSLSLGQRQ LLCLARALLT DAKILCIDEA
1381TASVDQKTDQ LLQQTICKRF ANKTVLTIAH RLNTILNSDR VLVLQAGRVV ELDSPATLRN
1441QPHSLFQQLL QSSQQGVPAS LGGP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1464 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.