LSBio
Rat TNF Alpha ELISA Kit (Sandwich ELISA)

Sandwich ELISA Platform Overview

Rat TNF Alpha ELISA Kit (Sandwich ELISA)

ELISA KitLSBio Guarantee
Reactivity:Rat
Detection MethodColorimetric
Format:96-Well Strip Plate
See all TNF Alpha antibodies →
$665.00each
Catalog Number: LS-F5193-1

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Overview

Description
TNF Alpha ELISA Kit LS-F5193 is a 96-Well enzyme-linked immunosorbent assay for the Quantitative detection of Rat TNF Alpha in Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates. It is a Sandwich assay which can detect TNF Alpha down to 15.6 pg/ml. For Research Use Only
Reactivity
Rt
Intended Use
Research Use Only
Category
ELISA Kits > Traditional ELISA Kits
Alternative Names
APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2

Assay Performance

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Sandwich Enzyme-Linked Immunosorbent Assay
Sensitivity
5.9 pg/ml
Detection Range
15.6 - 1000 pg/ml
Intra-Assay CV%
<10%
Inter-Assay CV%
<12%
Compatible Sample Types
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Detection Wavelength (Colorimetric)
450 nm
About This Kit
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Format

Format
96-Well Strip Plate
Kit Components
Coated 96-well Strip Plate, Adhesive Plate Sealers, Standard (Lyophilized), Sample Diluent, Detection Reagent A (Green), Detection Reagent B (Red), Assay Diluent A, Assay Diluent B, TMB Substrate, Stop Solution, Wash Buffer (30x)

Storage & Handling

Storage Conditions
Short term: 4°C; Long term: see manual.

Documentation

Manuals
Manual

The Potential of Asiatic Acid in the Reversion of Cyclophosphamide-Induced Hemorrhagic Cystitis in Rats. Andrzej Wróbel, Lukasz Zapala, Tomasz Kluz, Artur Rogowski, Marcin Misiek, Kajetan Juszczak, Jacek Sienko, Daniela Gold, Klaudia Stangel-Wójcikiewicz, Ewa Poleszak, Piotr Radziszewski. International journal of molecular sciences. 2021 May;22.

PubMed: 34072606 · PMC: PMC8198845

O-1602, an Agonist of Atypical Cannabinoid Receptors GPR55, Reverses the Symptoms of Depression and Detrusor Overactivity in Rats Subjected to Corticosterone Treatment. Andrzej Wróbel, Anna Serefko, Aleksandra Szopa, Daniela Ulrich, Ewa Poleszak, Tomasz Rechberger. Frontiers in pharmacology. 2020 August;11:1002.

PubMed: 32733244 · PMC: PMC7360849

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.