LSBio
Human KLB / Beta Klotho ELISA Kit (Sandwich ELISA)

Sandwich ELISA Platform Overview

Human KLB / Beta Klotho ELISA Kit (Sandwich ELISA)

Reactivity:Human
Detection MethodColorimetric
Format:96-Well Strip Plate
See all KLB / Beta Klotho antibodies →
$683.00each
Catalog Number: LS-F11894-1

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Overview

Description
KLB / Beta Klotho ELISA Kit LS-F11894 is a 96-Well enzyme-linked immunosorbent assay for the Quantitative detection of Human KLB / Beta Klotho in Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates. It is a Sandwich assay which can detect KLB / Beta Klotho down to 15.6 For Research Use Only
Reactivity
Hu
Intended Use
Research Use Only
Category
ELISA Kits > Traditional ELISA Kits
Alternative Names
BetaKlotho | Beta-klotho | BKL | KLB | Klotho beta | Klotho beta like | Klotho beta-like protein

Assay Performance

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Sandwich Enzyme-Linked Immunosorbent Assay
Sensitivity
10
Detection Range
15.6 - 1000 pg/ml
Intra-Assay CV%
<4.4%
Inter-Assay CV%
<7.9%
Compatible Sample Types
Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Detection Wavelength (Colorimetric)
450 nm
About This Kit
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Format

Format
96-Well Strip Plate
Kit Components
Coated 96-well Strip Plate, Standard (Lyophilized), Sample Diluent, Assay Diluent A, Assay Diluent B, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Adhesive Plate Sealers, Wash Buffer (25x)

Storage & Handling

Storage Conditions
Short term: 4°C; Long term: see manual.

Documentation

Manuals
Manual

The KLB rs17618244 gene variant is associated with fibrosing MAFLD by promoting hepatic stellate cell activation. Nadia Panera, Marica Meroni, Miriam Longo, Annalisa Crudele, Luca Valenti, Emanuele Bellacchio, Luca Miele, Valentina D'Oria, Erika Paolini, Marco Maggioni, Anna Ludovica Fracanzani, Anna Alisi, Paola Dongiovanni. EBioMedicine. 2021 February;65:103249.

PubMed: 33640795 · PMC: PMC7921469


ß-Klotho gene variation is associated with liver damage in children with NAFLD. Paola Dongiovanni, Annalisa Crudele, Nadia Panera, Ilaria Romito, Marica Meroni, Cristiano De Stefanis, Alessia Palma, Donatella Comparcola, Anna Ludovica Fracanzani, Luca Miele, Luca Valenti, Valerio Nobili, Anna Alisi. Journal of hepatology. 2020 March;72:411-419.

PubMed: 31655133

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.