LSBio
NAD/NADH Assay Kit (Fluorometric)

NAD/NADH Assay Kit (Fluorometric)

Assay FormatBiochemical Assay
Format:Kit
See all NAD/NADH antibodies →
$577.00each
Catalog Number: LS-K263-100

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Overview

Description
Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. This NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at lambda ex/em = 530/585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient Method to measure NAD, NADH and their ratio.Direct NAD/NADH Assays in 96-Well PlateNEW!!!
Target
NAD/NADH
Category
Assay Kits > All Assay Kits

Assay

Detection Method
Fluorescent / Fluorometric
Assay Type
Quantitative
Assay Format
Biochemical Assay
Compatible Sample Types
Cells, Tissue Homogenate

Format

Format
Kit
Kit Components
Assay Buffer, Enzyme A, Lactate, Enzyme B, Probe, NAD standard NAD\/NADH Extraction Buffers, NAD S Standard

Storage & Handling

Storage Conditions
Store at -20°C.

Documentation

Documents
Document

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.