LSBio
WNT5A Antibody (RPE)

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~42.3 kDa Wnt5a protein using Rabbit Anti-Wnt5a Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Wnt5a Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~42.3 kDa. Other Band(s): ~35 kDa isoform 2.

WNT5A Antibody (RPE)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human WNT5A antibodies →
$487.00each
Catalog Number: LS-C776433-100

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Overview

Description
WNT5A antibody LS-C776433 is an RPE-conjugated rabbit polyclonal antibody to human WNT5A. Validated for IHC and WB.
Application
IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
HWNT5A | Protein Wnt-5a | WNT-5A protein | WNT5A
Recommended Dilution: IHC
1:50
Recommended Dilution: WB
1:1000
UniProt Number
P41221
SwissProt
P41221

Target

Target
Human WNT5A
Antigen Species
Human
Epitope
Detects 42.3 kDa, 35 kDa. 35 kDa band is another isoform.
Gene Name
WNT5A

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide of Human Wnt5a (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Phycoerythrin
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.