LSBio
IHC-plus™ WNT5A Antibody (clone 6F2)

Anti-WNT5A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

IHC-plus™ WNT5A Antibody (clone 6F2)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$470.00each
Catalog Number: LS-B3859-50

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Overview

Description
WNT5A antibody LS-B3859 is an unconjugated mouse monoclonal antibody to human WNT5A. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
HWNT5A | Protein Wnt-5a | WNT-5A protein | WNT5A
Recommended Dilution: ELISA
1:10000
Recommended Dilution: IF/ICC
1:200 - 1:1000
Recommended Dilution: IHC-P
1:200 - 1:400
Recommended Dilution: WB
1:100 - 1:500
UniProt Number
P41221
SwissProt
P41221

Target

Target
WNT5A
Antigen Species
Human
Specificity
Human
Epitope
Human
Gene Name
WNT5A

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
6F2
Isotype
IgG1
Origin Species
Human

Immunogen

Immunogen
Purified recombinant fragment of WNT5A expressed in E. Coli.
Immunogen Type
Recombinant Protein Fragment

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Ascitic fluid, 0.03% sodium azide
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C.

Optimization, validation, and identification of two reliable antibodies for immunodetection of WNT5A. Prgomet Z, Andersson T, Lindberg P. Biotechnic & histochemistry : official publication of the Biological Stain Commission. 2017;92:46-58.

PubMed: 28157427


Differential Expression and Roles of Secreted Frizzled-Related Protein 5 and the Wingless Homolog Wnt5a in Periodontitis. Maekawa T, Kulwattanaporn P, Hosur K, Domon H, Oda M, Terao Y, Maeda T, Hajishengallis G. Journal of dental research. 2017 May;96:571-577.

PubMed: 28095260 · PMC: PMC5453495

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