Western blot analysis of extracts of various cell lines, using TSC2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Tuberin / TSC2 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C496666-20
Catalog Number: LS-C496666
For bulk orders or custom formulations:
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- Description
- TSC2 antibody LS-C496666 is an unconjugated rabbit polyclonal antibody to human TSC2 (Tuberin). Validated for IF, IHC and WB.
- Application
- IF, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- LAM | Tuberous sclerosis 2 protein | TSC4 | Tuberin | Tuberous sclerosis 2 | TSC2
- Recommended Dilution: IF/ICC
- 1:50 - 1:100
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:200 - 1:500
- UniProt Number
- P49815
- NCBI GenBank
- NM_000548 | NP_000539.2
- SwissProt
- P49815
Target
- Target
- Human Tuberin / TSC2
- Antigen Species
- Human
- Gene Name
- TSC2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A synthetic peptide of human TSC2
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C, avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS