Detection of Mouse KAP-1 by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 mu M etoposide for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
TRIM28 / KAP1 Antibody (aa175-225)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunoprecipitation, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C290791-10
Catalog Number: LS-C290791
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- Description
- KAP1 antibody LS-C290791 is an unconjugated rabbit polyclonal antibody to mouse KAP1 (TRIM28) (aa175-225). Validated for IP and WB.
- Application
- IP, WB
- Reactivity
- Ms
- Predicted Reactivity
- Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- E3 SUMO-protein ligase TRIM28 | KRIP-1 | Nuclear corepressor KAP-1 | KAP-1 | KAP1 | KRAB-interacting protein 1 | TIF1B | TRIM28 | RNF96 | TIF1-beta | TIF1beta | Tripartite motif-containing 28 | KRAB-associated protein 1 | RING finger protein 96 | TF1B | Tif1beta zinc finger protein | Tripartite motif containing 28
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- Q13263
- NCBI GenBank
- NM_005762 | NP_005753.1
- SwissProt
- Q13263
Target
- Target
- Mouse TRIM28 / KAP1
- Antigen Species
- Mouse
- Epitope
- Region between residue 175 to 225 of mouse Tripartite Motif-Containing 28 using the numbering given in entry NP_035718.2 (GeneID 21849).
- Gene Name
- TRIM28
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 21849 corresponding to amino acids between 175 and 225 of UniprotID: Q62318
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate