Detection of Human KAP1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug), and 293T (50 ug) cells. Antibodies: Affinity purified rabbit anti-KAP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
TRIM28 / KAP1 Antibody (aa1-50)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C287177-10
Catalog Number: LS-C287177
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- Description
- KAP1 antibody LS-C287177 is an unconjugated rabbit polyclonal antibody to KAP1 (TRIM28) (aa1-50) from human. It is reactive with human and mouse. Validated for ICC, IHC, IP and WB. Cited in 1 publication.
- Application
- ICC, IHC, IP, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- E3 SUMO-protein ligase TRIM28 | KRIP-1 | Nuclear corepressor KAP-1 | KAP-1 | KAP1 | KRAB-interacting protein 1 | TIF1B | TRIM28 | RNF96 | TIF1-beta | TIF1beta | Tripartite motif-containing 28 | KRAB-associated protein 1 | RING finger protein 96 | TF1B | Tif1beta zinc finger protein | Tripartite motif containing 28
- Recommended Dilution: IF/ICC
- 1:500 - 1:2500
- Recommended Dilution: IHC
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- Q13263
- NCBI GenBank
- NM_005762 | NP_005753.1
- SwissProt
- Q13263
Target
- Target
- Human TRIM28 / KAP1
- Antigen Species
- Human
- Epitope
- Region between residue 1 and 50 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_005753.1 (GeneID 10155).
- Gene Name
- TRIM28
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 10155 corresponding to amino acids between 1 and 50 of UniprotID: Q13263
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate
Absolute Quantification of Transcription Factors Reveals Principles of Gene Regulation in Erythropoiesis. Mark A Gillespie, Carmen G Palii, Daniel Sanchez-Taltavull, Paul Shannon, William J R Longabaugh, Damien J Downes, Karthi Sivaraman, Herbert M Espinoza, Jim R Hughes, Nathan D Price, Theodore J Perkins, Jeffrey A Ranish, Marjorie Brand. Molecular cell. 2020 June;78:960-974.e11. PubMed: 32330456 PMC: PMC7344268