Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug TRIM25 antibody. Western blot was performed from the immunoprecipitate using TRIM25 antibody at a dilition of 1:1000.

TRIM25 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunoprecipitation, Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$281.00each

Catalog Number: LS-C748006-20

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Overview

Description: TRIM25 antibody LS-C748006 is an unconjugated rabbit polyclonal antibody to TRIM25 from human. It is reactive with human and mouse. Validated for IP and WB.
Application: IP, WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: EFP | Zinc finger protein 147 | Z147 | Zinc finger protein-147 | ZNF147 | Tripartite motif containing 25 | RING finger protein 147 | RNF147 | TRIM25 | Tripartite motif-containing 25
Recommended Dilution: IP: 1:50 - 1:100
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q14258
NCBI GenBank: NM_005082 | NP_005073.2
SwissProt: Q14258

Target

Target: Human TRIM25
Antigen Species: Human
Gene Name: TRIM25

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM25 (NP_005073.2). SPNAQVACDHCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEPTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALP
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)