Anti-Mouse RANKL Antibody - Western Blot. Anti-mouse RANKL antibody in western blot shows detection of recombinant mouse RANKL raised in E. coli. Recombinant truncated protein (0.1 ug, 19.9 kD) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse RANKL antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
TNFSF11 / RANKL / TRANCE Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Mouse |
| Format: | Lyophilized |
$579.00each
Catalog Number: LS-C154136-100
Catalog Number: LS-C154136
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- Description
- TRANCE antibody LS-C154136 is an unconjugated rabbit polyclonal antibody to TRANCE (TNFSF11 / RANKL) from mouse. It is reactive with mouse and rat. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Ms
- Predicted Reactivity
- Hamster, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD254 | CD254 antigen | HRANKL2 | OPTB2 | TRANCE | TNFSF11 | ODF | OPGL | SOdf | Osteoprotegerin ligand | RANKL
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- O14788
- NCBI GenBank
- AAC39731.1 | AF053712
- SwissProt
- O14788
Target
- Target
- Mouse TNFSF11 / RANKL / TRANCE
- Antigen Species
- Mouse
- Epitope
- This antibody is specific for mouse RANKL protein. A BLAST analysis was used to suggest cross-reactivity with RANKL from mouse sources based on 100% homology with the immunizing sequence. Based on 97% homology, cross-reactivity with rat, 91% with Chinese hamster, 90% with Thirteen-lined ground squirrel; other sources has not been determined.
- Gene Name
- TNFSF11
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse RANKL protein (aa 143-316) produced in E.coli.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
- Concentration
- 1 mg/mL
- Preservative
- No
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl