LSBio
TNF Alpha Antibody (Liquid)

Western blot using the protein-A purified anti-swine TNFa antibody shows detection of recombinant Swine TNFa at about 16.9 kDa raised in yeast. The identity of the faint band at 135 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 µg/mL primary antibody overnight at 4°C. After washing, membrane was probed with for 45 min at room temperature.

TNF Alpha Antibody (Liquid)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Pig
Format:Liquid
See all Pig TNF Alpha antibodies →
$311.00each
Catalog Number: LS-C745272-25

For bulk orders or custom formulations:

Request a Quote

Overview

Description
TNF Alpha antibody LS-C745272 is an unconjugated rabbit polyclonal antibody to pig TNF Alpha. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Por
Predicted Reactivity
NHP
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Recommended Dilution: ELISA
1:2000 - 1:2500
Recommended Dilution: WB
1:1000
UniProt Number
P01375
NCBI GenBank
NM_000594, NP_000585.2
SwissProt
P01375

Target

Target
Pig TNF Alpha
Antigen Species
Por
Epitope
A BLAST analysis was used to suggest cross-reactivity with TNFa from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against TNFa from baboon based on 90% homology. Cross-reactivity with TNFa from sources other than swine has not been determined.
Gene Name
TNF

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 154 amino acids of mature swine TNFa.
Immunogen Type
Recom

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1.15 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Prot A

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.