Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-Swine TNFa Antibody - Western Blot. Western blot of protein-A purified anti-swine TNFa antibody shows detection of recombinant swine TNFa at 16.9 kD, raised in yeast. The identity of the faint band at 135 kD is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 ug/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG ( for 45 min at room temperature.
Polyclonal Rabbit anti-Pig TNF Alpha Antibody (Lyophilized, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Pig |
| Format: | Lyophilized |
$579.00each
Catalog Number: LS-C154152-100
Catalog Number: LS-C154152
For bulk orders or custom formulations:
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- Description
- TNF Alpha antibody LS-C154152 is an unconjugated rabbit polyclonal antibody to pig TNF Alpha. Validated for WB.
- Application
- WB
- Reactivity
- Por
- Predicted Reactivity
- Non-Human Primate
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P01375
- NCBI GenBank
- NM_000594 | NP_000585.2
- SwissProt
- P01375
Target
- Target
- Pig TNF Alpha
- Antigen Species
- Pig
- Epitope
- A BLAST analysis was used to suggest cross-reactivity with TNFa from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against TNFa from baboon based on 90% homology. Cross-reactivity with TNFa from sources other than swine has not been determined.
- Gene Name
- TNF
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 154 amino acids of mature swine TNFa.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
