Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human large intestine tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
TJP2 / ZO2 / ZO-2 Antibody (aa740-790)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general) |
| Reactivity: | Human |
| Format: | Lyophilized |
$401.00each
Catalog Number: LS-C483078-100
Catalog Number: LS-C483078
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- Description
- ZO-2 antibody LS-C483078 is an unconjugated rabbit polyclonal antibody to human ZO-2 (TJP2 / ZO2) (aa740-790). Validated for IHC.
- Application
- IHC
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C9DUPq21.11 | DUP9q21.11 | DFNA51 | TJP2 | ZO-2 | Zona occludens 2 | Zonula occludens protein 2 | Tight junction protein 2 | Tight junction protein ZO-2 | ZO2 | X104 | Zona occludens protein 2
- UniProt Number
- Q9UDY2
- NCBI GenBank
- NM_004817 | NP_004808.2
- SwissProt
- Q9UDY2
Target
- Target
- Human TJP2 / ZO2 / ZO-2
- Antigen Species
- Human
- Epitope
- Specific for ZO2.
- Gene Name
- TJP2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Synthetic peptide from aa740-790 of human ZO2 conjugated to an immunogenic carrier protein was used as the antigen. The peptide shares 98% identity with mouse and rat sequences.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.