Detection of human THEMIS by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THEMIS antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
THEMIS / SPOT Antibody (aa591-641)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C762793-10
Catalog Number: LS-C762793
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- Description
- SPOT antibody LS-C762793 is an unconjugated rabbit polyclonal antibody to human SPOT (THEMIS) (aa591-641). Validated for IP and WB.
- Application
- IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C6orf207 | C6orf190 | BA325O24.3 | GRB2-associated protein | Protein THEMIS | SPOT | Thymocyte selection associated | BA325O24.4 | TSEPA | GASP | THEMIS
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- Q8BGW0
- NCBI GenBank
- NP_848781.1
- SwissProt
- Q8BGW0
Target
- Target
- Human THEMIS / SPOT
- Antigen Species
- Human
- Gene Name
- Themis
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Mouse
Immunogen
- Immunogen
- A portion of GeneID: 387357 corresponding to ammino acids between 591 and 641 of UniprotID: Q8N1K5
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 year.
- Recommended Storage Buffer
- Tris-citrate