LSBio
Human THEMIS / SPOT Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human THEMIS / SPOT Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all THEMIS antibodies →
$220.00each
Catalog Number: LS-G94066-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human THEMIS (NM_001164685) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C6orf207 | C6orf190 | BA325O24.3 | GRB2-associated protein | Protein THEMIS | SPOT | Thymocyte selection associated | BA325O24.4 | TSEPA | GASP | THEMIS
Origin Species
Human
SwissProt
Q8BGW0

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MALSLEEFVH SLDLRTLPRV LEIQAGIYLE GSIYEMFGNE CCFSTGEVIK ITGLKVKKII
61AEICEQIEGC ESLQPFELPM NFPGLFKIVA DKTPYLTMEE ITRTIHIGPS RLGHPCFYHQ
121KDIKLENLII KQGEQIMLNS VEEIDGEIMV SCAVARNHQT HSFNLPLSQE GEFYECEDER
181IYTLKEIVEW KIPKNRTRTV NLTDFSNKWD STNPFPKDFY GTLILKPVYE IQGVMKFRKD
241IIRILPSLDV EVKDITDSYD ANWFLQLLST EDLFEMTSKE FPIVTEVIEA PEGNHLPQSI
301LQPGKTIVIH KKYQASRILA SEIRSNFPKR HFLIPTSYKG KFKRRPREFP TAYDLEIAKS
361EKEPLHVVAT KAFHSPHDKL SSVSVGDQFL VHQSETTEVL CEGIKKVVNV LACEKILKKS
421YEAALLPLYM EGGFVEVIHD KKQYPISELC KQFRLPFNVK VSVRDLSIEE DVLAATPGLQ
481LEEDITDSYL LISDFANPTE CWEIPVGRLN MTVQLVSNFS RDAEPFLVRT LVEEITEEQY
541YMMRRYESSA SHPPPRPPKH PSVEETKLTL LTLAEERTVD LPKSPKAGVQ WRDLGSLQPL
601PPGFKQFSAS ASHVAGITGT PHHVQRHHVD ITKKLHPNQA GLDSKVLIGS QNDLVDEEKE
661RSNRGATAIA ETFKNEKHQK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

680 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.