Western blot analysis of extracts of various cell lines, using TGFBR1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
TGFBR1 / ALK5 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C408240-20
Catalog Number: LS-C408240
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- Description
- ALK5 antibody LS-C408240 is an unconjugated rabbit polyclonal antibody to human ALK5 (TGFBR1). Validated for WB.
- Application
- WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AAT5 | ACVRLK4 | ALK-5 | ALK5 | Activin receptor-like kinase 5 | LDS1A | LDS2A | MSSE | SKR4 | TGF beta receptor I | TGF-beta receptor type-1 | TGF-beta type I receptor | TbetaR-I | TGFR-1 | TGF-beta receptor type I | TGFBR1
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P36897
- NCBI GenBank
- NM_004612 | NP_004603.1
- SwissProt
- P36897
Target
- Target
- Human TGFBR1 / ALK5
- Antigen Species
- Human
- Epitope
- Human TGFBR1 / ALK5
- Gene Name
- TGFBR1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 34-124 of human TGFBR1 (NP_001124388.1). LQCFCHLCTKDNFTCVTDGLCFVSVTETTDKVIHNSMCIAEIDLIPRDRPFVCAPSSKTGSVTTTYCCNQDHCNKIELPTTGLPLLVQRTI
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS