Immunofluorescence staining of TCF12 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TCF12 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).
TCF12 / HEB Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815725-100
Catalog Number: LS-C815725
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- Description
- HEB antibody LS-C815725 is an unconjugated rabbit polyclonal antibody to human HEB (TCF12). Validated for ICC, IF, IHC and WB.
- Application
- ICC, IF, IHC-P, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BHLHb20 | E-box-binding protein | HEB | HsT17266 | HTF4 | TCF-12 | TCF12 | Transcription factor 12 | Transcription factor HTF-4 | DNA-binding protein HTF4
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: IHC-P
- 1:200 - 1:1000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q99081
- NCBI GenBank
- NM_003205 | NP_003196.1
- SwissProt
- Q99081
Target
- Target
- Human TCF12 / HEB
- Antigen Species
- Human
- Epitope
- Human TCF12
- Gene Name
- TCF12
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the N-Terminus of Human TCF12
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.0, 0.03% Proclin 300
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS