Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TCF12 / HEB Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102604-100
Catalog Number: LS-G102604
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TCF12 (NM_207036), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BHLHb20 | E-box-binding protein | HEB | HsT17266 | HTF4 | TCF-12 | TCF12 | Transcription factor 12 | Transcription factor HTF-4 | DNA-binding protein HTF4
- Origin Species
- Human
- NCBI GenBank
- NM_003205
- SwissProt
- Q99081
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNPQQQRMAA IGTDKELSDL LDFSAMFSPP VNSGKTRPTT LGSSQFSGSG IDERGGTTSW61GTSGQPSPSY DSSRGFTDSP HYSDHLNDSR LGAHEGLSPT PFMNSNLMGK TSERGSFSLY121SRDTGLPGCQ SSLLRQDLGL GSPAQLSSSG KPGTAYYSFS ATSSRRRPLH DSAALDPLQA181KKVRKVPPGL PSSVYAPSPN SDDFNRESPS YPSPKPPTSM FASTFFMQDG THNSSDLWSS241SNGMSQPGFG GILGTSTSHM SQSSSYGNLH SHDRLSYPPH SVSPTDINTS LPPMSSFHRG301STSSSPYVAA SHTPPINGSD SILGTRGNAA GSSQTGDALG KALASIYSPD HTSSSFPSNP361STPVGSPSPL TGTSQWPRPG GQAPSSPSYE NSLHSLKNRV EQQLHEHLQD AMSFLKDVCE421QSRMEDRLDR LDDAIHVLRN HAVGPSTSLP AGHSDIHSLL GPSHNAPIGS LNSNYGGSSL481VASSRSASMV GTHREDSVSL NGNHSVLSST VTTSSTDLNH KTQENYRGGL QSQSGTVVTT541EIKTENKEKD ENLHEPPSSD DMKSDDESSQ KDIKVSSRGR TSSTNEDEDL NPEQKIEREK601ERRMANNARE RLRVRDINEA FKELGRMCQL HLKSEKPQTK LLILHQAVAV ILSLEQQVRE661RNLNPKAACL KRREEEKVSA VSAEPPTTLP GTHPGLSETT NPMGHMSGPSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
709 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.