Western blot analysis of extracts of HeLa cells, using Phospho-TBC1D4-S588 antibody at 1:2000 dilution. HeLa cells were treated by IGF-1 (50ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 30s.
TBC1D4 / AS160 Antibody (phospho-Ser588)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$293.00each
Catalog Number: LS-C750595-20
Catalog Number: LS-C750595
For bulk orders or custom formulations:
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- Description
- AS160 antibody LS-C750595 is an unconjugated rabbit polyclonal antibody to AS160 (TBC1D4) (pSer588) from human. It is reactive with human, mouse and rat. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Akt substrate of 160 kDa | AS160 | KIAA0603 | TBC1 domain family, member 4 | TBC1 domain family member 4 | TBC1D4
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- O60343
- NCBI GenBank
- AB011175 | BAA25529.2
- SwissProt
- O60343
Target
- Target
- Human TBC1D4 / AS160
- Antigen Species
- Human
- Gene Name
- TBC1D4
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS