Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TBC1D4 / AS160 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113703-100
Catalog Number: LS-G113703
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBC1D4 (NM_014832), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Akt substrate of 160 kDa | AS160 | KIAA0603 | TBC1 domain family | member 4 | TBC1 domain family member 4 | TBC1D4
- Origin Species
- Human
- NCBI GenBank
- AB011175
- SwissProt
- O60343
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 146.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPPSCIQDE PFPHPLEPEP GVSAQPGPGK PSDKRFRLWY VGGSCLDHRT TLPMLPWLMA61EIRRRSQKPE AGGCGAPAAR EVILVLSAPF LRCVPAPGAG ASGGTSPSAT QPNPAVFIFE121HKAQHISRFI HNSHDLTYFA YLIKAQPDDP ESQMACHVFR ATDPSQVPDV ISSIRQLSKA181AMKEDAKPSK DNEDAFYNSQ KFEVLYCGKV TVTHKKAPSS LIDDCMEKFS LHEQQRLKIQ241GEQRGPDPGE DLADLEVVVP GSPGDCLPEE ADGTDTHLGL PAGASQPALT SSRVCFPERI301LEDSGFDEQQ EFRSRCSSVT GVQRRVHEGS QKSQPRRRHA SAPSHVQPSD SEKNRTMLFQ361VGRFEINLIS PDTKSVVLEK NFKDISSCSQ GIKHVDHFGF ICRESPEPGL SQYICYVFQC421ASESLVDEVM LTLKQAFSTA AALQSAKTQI KLCEACPMHS LHKLCERIEG LYPPRAKLVI481QRHLSSLTDN EQADIFERVQ KMKPVSDQEE NELVILHLRQ LCEAKQKTHV HIGEGPSTIS541NSTIPENATS SGRFKLDILK NKAKRSLTSS LENIFSRGAN RMRGRLGSVD SFERSNSLAS601EKDYSPGDSP PGTPPASPPS SAWQTFPEED SDSPQFRRRA HTFSHPPSST KRKLNLQDGR661AQGVRSPLLR QSSSEQCSNL SSVRRMYKES NSSSSLPSLH TSFSAPSFTA PSFLKSFYQN721SGRLSPQYEN EIRQDTASES SDGEGRKRTS STCSNESLSV GGTSVTPRRI SWRQRIFLRV781ASPMNKSPSA MQQQDGLDRN ELLSLSPLSP TMEEEPLVVF LSGEDDPEKI EERKKSKELR841SLWRKAIHQQ ILLLRMEKEN QKLEGASRDE LQSRKVKLDY EEVGACQKEV LITWDKKLLN901CRAKIRCDME DIHTLLKEGV PKSRRGEIWQ FLALQYRLRH RLPNKQQPPD ISYKELLKQL961TAQQHAILVD LGRTFPTHPY FSVQLGPGQL SLFNLLKAYS LLDKEVGYCQ GISFVAGVLL1021LHMSEEQAFE MLKFLMYDLG FRKQYRPDMM SLQIQMYQLS RLLHDYHRDL YNHLEENEIS1081PSLYAAPWFL TLFASQFSLG FVARVFDIIF LQGTEVIFKV ALSLLSSQET LIMECESFEN1141IVEFLKNTLP DMNTSEMEKI ITQVFEMDIS KQLHAYEVEY HVLQDELQES SYSCEDSETL1201EKLERANSQL KRQNMDLLEK LQVAHTKIQA LESNLENLLT RETKMKSLIR TLEQEKMAYQ1261KTVEQLRKLL PADALVNCDL LLRDLNCNPN NKAKIGNKPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1299 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.