Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western Blot of rabbit anti-STAT2pS734 antibody. Lane 1: U6A cells (STAT2 deficient) untreated. Lane 2: U6A cells reconstituted with STAT2 untreated. Lane 3: U6A cells, STAT2 treated with IFNa (1h). Lane 4: U6A cells, STAT2 + IFNa (4h). Lane 5: U6A cells, STAT2 + IFNa (8h). Lane 6: U6A cells reconstituted with S734A-STAT2 mutant untreated. Lane 7: U6A cells, S734A-STAT2 treated with IFNa (1h). Lane 8: U6A cells, S734A-STAT2 + IFNa (4h). Lane 9: U6A cells, S734A-STAT2 + IFNa (8h). Load: 20 µg per lane. Primary antibody: STAT2 pS734 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 97.9 kDa, ~110 kDa for STAT2pS734. Other band(s): Non specific 100kDa, STAT2, GAPDH loading control.
STAT2 Antibody (phospho-Ser734)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Non-Human Primate |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745435-25
Catalog Number: LS-C745435
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- STAT2 antibody LS-C745435 is an unconjugated rabbit polyclonal antibody to STAT2 (pSer734) from human. It is reactive with human and monkey. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, NHP
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ISGF-3 | STAT113 | STAT2 | p113
- Recommended Dilution: ELISA
- 1:5000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P52630
- NCBI GenBank
- NM_005419, NP_005410.1
- SwissProt
- P52630
Target
- Target
- Human STAT2
- Antigen Species
- Hu
- Epitope
- This antibody is specific for phosphorylated S734 human STAT2. A BLAST analysis was used to suggest cross-reactivity with STAT2 pS734 protein from human and monkey sources based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
- Gene Name
- STAT2
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- STAT2 pS734 antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to serine 734 a region near the C-Terminus of human STAT2 protein.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.44 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
