LSBio
STAT2 Antibody (phospho-Ser734)

Western Blot of Rabbit anti-STAT2pS734 antibody. Lane 1: U6A cells (STAT2 deficient) untreated. Lane 2: U6A cells reconstituted with STAT2 untreated. Lane 3: U6A cells, STAT2 treated with IFNa (1h). Lane 4: U6A cells, STAT2 + IFNa (4h). Lane 5: U6A cells, STAT2 + IFNa (8h). Lane 6: U6A cells reconstituted with S734A-STAT2 mutant untreated. Lane 7: U6A cells, S734A-STAT2 treated with IFNa (1h). Lane 8: U6A cells, S734A-STAT2 + IFNa (4h). Lane 9: U6A cells, S734A-STAT2 + IFNa (8h). Load: 20 µg per lane. Primary antibody: STAT2 pS734 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800® rabbit secondary antibody at 1:10,000 for 45 min at RT.

STAT2 Antibody (phospho-Ser734)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human, Non-Human Primate
Format:Liquid
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$579.00each
Catalog Number: LS-C209345-100

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Overview

Description
STAT2 antibody LS-C209345 is an unconjugated rabbit polyclonal antibody to STAT2 (pSer734) from human. It is reactive with human and monkey. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu, NHP
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ISGF-3 | STAT113 | STAT2 | p113
Recommended Dilution: ELISA
1:5000
Recommended Dilution: WB
1:1000
UniProt Number
P52630
SwissProt
P52630

Target

Target
Human STAT2
Antigen Species
Human
Epitope
This antibody is specific for phosphorylated S734 human STAT2. A BLAST analysis was used to suggest cross-reactivity with STAT2 pS734 protein from human and monkey sources based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Gene Name
STAT2

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
STAT2 pS734 antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to serine 734 a region near the C-Terminus of human STAT2 protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
0.64 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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