Western blot analysis of extracts of HeLa cells, using SPOP antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.

SPOP Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Rat
Format:	Liquid

$281.00each

Catalog Number: LS-C747292-20

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: SPOP antibody LS-C747292 is an unconjugated rabbit polyclonal antibody to SPOP from human. It is reactive with human and rat. Validated for WB.
Application: WB
Reactivity: Hu, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: BTBD32 | HIB homolog 1 | TEF2 | Roadkill homolog 1 | Speckle-type POZ protein | SPOP
Recommended Dilution: WB: 1:500 - 1:1000
UniProt Number: O43791
NCBI GenBank: NP_003554.1
SwissProt: O43791

Target

Target: Human SPOP
Antigen Species: Human
Gene Name: SPOP

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 175-374 of human SPOP (NP_003554.1). TMNMVKVPECRLADELGGLWENSRFTDCCLCVAGQEFQAHKAILAARSPVFSAMFEHEMEESKKNRVEINDVEPEVFKEMMCFIYTGKAPNLDKMADDLLAAADKYALERLKVMCEDALCSNLSVENAAEILILADLHSADQLKTQAVDFINYHASDVLETSGWKSMVVSHPHLVAEAYRSLASAQCPFLGPPRKRLKQS
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)