Formalin-fixed, paraffin-embedded human Lymph Node stained with CD43 Mouse Recombinant Monoclonal Antibody (rSPN/839).
SPN / CD43 Antibody (clone rSPN/839)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Recombinant Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$379.00each
Catalog Number: LS-C751368-20
Catalog Number: LS-C751368
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- Description
- CD43 antibody LS-C751368 is an unconjugated mouse recombinant monoclonal antibody to human CD43 (SPN). Validated for Flow, IF, IHC and WB.
- Application
- FC, IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Recombinant Antibodies
- Alternative Names
- GPL115 | Leukosialin | Leukocyte sialoglycoprotein | Galactoglycoprotein | GALGP | Sialophorin | LSN | CD43 | CD43 antigen | SPN
- Recommended Dilution: FC
- 0.5 - 1 µg/10E6 cells
- Recommended Dilution: IF/ICC
- 1 - 2 µg/ml
- Recommended Dilution: IHC-P
- 0.5 - 1 µg/ml
- Recommended Dilution: WB
- 1 - 2 µg/ml
- UniProt Number
- P16150
- NCBI GenBank
- NM_003123 | NP_003114.1
- SwissProt
- P16150
Target
- Target
- Human SPN / CD43
- Antigen Species
- Human
- Epitope
- It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. Epitope of MAb Bra7G is clearly different from that of MAb rSPN/839, called "b" as opposed to "a" for rSPN/839. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
- Gene Name
- SPN
Antibody
- Host Species
- Mouse
- Clonality
- Recombinant Monoclonal
- Clone
- rSPN/839
- Isotype
- IgG1
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant full-length human CD43 protein. Antigen Molecular Weight: 95, 115, or 135 kDa
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
- Concentration
- 200 µg/mL
- Preservative
- Yes
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 2 years.
- Recommended Storage Buffer
- 10 mM PBS