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PathPlus™ SPN / CD43 Antibody (clone SPN/1766R)

Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)

PathPlus™ SPN / CD43 Antibody (clone SPN/1766R)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Recombinant Monoclonal
Applications:Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all SPN / CD43 antibodies →
$536.00each
Catalog Number: LS-B16299-100

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Overview

Description
CD43 antibody LS-B16299 is an unconjugated rabbit recombinant monoclonal antibody to human CD43 (SPN). Validated for Flow, IF, IHC and WB.
Application
FC, IF, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Recombinant Antibodies
Alternative Names
GPL115 | Leukosialin | Leukocyte sialoglycoprotein | Galactoglycoprotein | GALGP | Sialophorin | LSN | CD43 | CD43 antigen | SPN
Recommended Dilution: FC
0.5 - 1 µg/10E6 cells
Recommended Dilution: IF/ICC
1 - 2 µg/ml
Recommended Dilution: IHC-P
1:100
Recommended Dilution: WB
1 - 2 µg/ml
UniProt Number
P16150
NCBI GenBank
NM_003123 | NP_003114.1
SwissProt
P16150

Target

Target
SPN / CD43
Antigen Species
Human
Specificity
It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Epitope
It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Gene Name
SPN

Antibody

Host Species
Rabbit
Clonality
Recombinant Monoclonal
Clone
SPN/1766R
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
Recombinant full-length human SPN protein. Antigen Molecular Weight: 95, 115, or 135 kDa
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Concentration
200 µg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Protein A/G Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 2 years.
Recommended Storage Buffer
10 mM PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.