Detection of Human CD43 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD43 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
SPN / CD43 Antibody (aa350-400)
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| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C291031-10
Catalog Number: LS-C291031
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- Description
- CD43 antibody LS-C291031 is an unconjugated rabbit polyclonal antibody to human CD43 (SPN) (aa350-400). Validated for IHC, IP and WB.
- Application
- IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- GPL115 | Leukosialin | Leukocyte sialoglycoprotein | Galactoglycoprotein | GALGP | Sialophorin | LSN | CD43 | CD43 antigen | SPN
- Recommended Dilution: IHC
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- P16150
- NCBI GenBank
- NM_003123 | NP_003114.1
- SwissProt
- P16150
Target
- Target
- Human SPN / CD43
- Antigen Species
- Human
- Epitope
- Region between residue 350 to 400 of human CD43 Molecule using the numbering given in entry NP_001025459.1 (GeneID 6693).
- Gene Name
- SPN
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 6693 corresponding to amino acids between 350 and 400 of UniprotID: P16150
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate