Detection of human Phospho SMC3 (S1083) by western blot (WB). Samples: Whole cell lysate (50 µg) from HeLa cells treated with 100 µM etoposide and/or 100 µM phosphatase for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho SMC3 (S1083) antibodyused for WB at 1 µg/ml (upper panel); to detect total SMC3, rabbit anti-SMC3 was used for WB at 0.1 µg/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 10 seconds (lower panel).
SMC3 / HCAP Antibody (phospho-Ser1083)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C762158-10
Catalog Number: LS-C762158
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- Description
- HCAP antibody LS-C762158 is an unconjugated rabbit polyclonal antibody to human HCAP (SMC3) (pSer1083). Validated for IP and WB.
- Application
- IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Non-Human Primate, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Bamacan | CDLS3 | CSPG6 | HCAP | SMC-3 | SMC3 | BAM | BMH | SMC protein 3 | SMC3L1
- Recommended Dilution: WB
- 1:500 - 1:2500
- UniProt Number
- Q9UQE7
- NCBI GenBank
- NM_005445 | NP_005436.1
- SwissProt
- Q9UQE7
Target
- Target
- Human SMC3 / HCAP
- Antigen Species
- Human
- Gene Name
- SMC3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A portion of GeneID: 9126 corresponding to ammino acids surrounding phospho-serine 1083 of UniprotID: Q9UQE7
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 year.
- Recommended Storage Buffer
- Tris-citrate