Chromatin Immunoprecipitation Hela (1.1*10E6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-BRM or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (SMARCA2) against the ESR1 pS2 promoter.
SMARCA2 / BRM Antibody (aa700-1216)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Chromatin Immunoprecipitation, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general) |
| Reactivity: | Human |
| Format: | Liquid |
$300.00each
Catalog Number: LS-C370344-50
Catalog Number: LS-C370344
For bulk orders or custom formulations:
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- Description
- BRM antibody LS-C370344 is an unconjugated rabbit polyclonal antibody to human BRM (SMARCA2) (aa700-1216). Validated for ChrIP and ELISA.
- Application
- ChIP, ELISA, IHC
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BAF190 | BRG1-associated factor 190B | BRM | HSNF2a | HBRM | NCBRS | SNF2/SWI2-like protein 2 | SMARCA2 | SNF2A | SWI2 | SNF2 | SNF2L2 | ATP-dependent helicase SMARCA2 | BAF190B | Protein brahma homolog | SNF2-alpha | SNF2LA | Sth1p
- UniProt Number
- P51531
- NCBI GenBank
- NM_003070 | NP_003061.3
- SwissProt
- P51531
Target
- Target
- Human SMARCA2 / BRM
- Antigen Species
- Human
- Epitope
- Human SMARCA2 / BRM
- Gene Name
- SMARCA2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant human Probable global transcription activator SNF2L2 protein (aa700-1216).
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- Yes
- Purification Method
- Caprylic Acid / Ammonium Sulfate Precipitation
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS