Western blot of lysates from 293, HepG2 cell line (from left to right), using SIRT3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
SIRT3 / Sirtuin 3 Antibody (aa250-279)
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
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- Description
- Sirtuin 3 antibody LS-C100489 is an unconjugated rabbit polyclonal antibody to Sirtuin 3 (SIRT3) (aa250-279) from human. It is reactive with human and mouse. Validated for IHC and WB. Cited in 2 publications.
- Application
- IHC, IHC-P, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HSIRT3 | Sir2-like 3 | SIR2-like protein 3 | SIRT3 | SIR2L3 | Sirtuin 3 | Sirtuin type 3
- Recommended Dilution: IHC-P
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q9NTG7
- NCBI GenBank
- NM_012239 | NP_036371.1
- SwissProt
- Q9NTG7
Target
- Target
- Human SIRT3 / Sirtuin 3
- Antigen Species
- Human
- Epitope
- This SIRT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the C-terminal region of human SIRT3.
- Gene Name
- SIRT3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS
FoxO1 mediates an autofeedback loop regulating SIRT1 expression. Xiong S, Salazar G, Patrushev N, Alexander RW. The Journal of biological chemistry. 2011;286:5289-99.
PubMed: 21149440 · PMC: PMC3037641
Integration of -catenin, sirtuin, and FOXO signaling protects from mutant huntingtin toxicity. Parker JA, Vazquez-Manrique RP, Tourette C, Farina F, Offner N, Mukhopadhyay A, Orfila AM, Darbois A, Menet S, Tissenbaum HA, Neri C. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2012;32:12630-40.
PubMed: 22956852