Western blot analysis of extracts of various cell lines, using ROCK1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Rho Kinase / ROCK1 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

See all Human Rho Kinase / ROCK1 antibodies â†’

$293.00each

Catalog Number: LS-C408278-20

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Overview

Description: ROCK1 antibody LS-C408278 is an unconjugated rabbit polyclonal antibody to ROCK1 (Rho Kinase) from human. It is reactive with human, mouse and rat. Validated for IHC, IP and WB.
Application: IHC, IP, WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: p160 Rho-associated kinase | p160ROCK | p160 ROCK-1 | p160-ROCK | p160RhoK | Rho-associated kinase | ROCK1 | RockI | Rok | Rok alpha | Rho-alpha kinase | p160 rho-kinase | Rho kinase | ROCK-I | Rokalpha
Recommended Dilution: IHC: 1:50 - 1:100
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q13464
NCBI GenBank: NM_005406 | NP_005397.1
SwissProt: Q13464

Target

Target: Human Rho Kinase / ROCK1
Antigen Species: Human
Epitope: Human Rho Kinase / ROCK1
Gene Name: ROCK1

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1). YSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLN
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)