Monoclonal Rat anti-Mouse CD90.2 (Mouse) Antibody (clone 30H12, AF647)
Catalog Number: LS-C810655
2 sizes / from $259.00
Antibody: Rat Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse
Format: Liquid
Detection of cleaved ROCK-1 in apoptotic Jurkat cell lysates (10 µg/lane). Lane 1. Jurkat cells were treated with camptothecin. Lane 2. Jurkat cells were treated with anti-Fas antibody (10 ng/ml for 2 hrs). antibody was used at a concentration of 1 ug/ml.
Monoclonal Mouse anti-Human Rho Kinase / ROCK1 Antibody (clone 154C1465, aa1114-1126, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Bovine, Dog, Horse, Human, Mammal (Other), Mouse, Non-Human Primate, Rat |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C511-0.1
Catalog Number: LS-C511
For bulk orders or custom formulations:
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- Description
- ROCK1 antibody LS-C511 is an unconjugated mouse monoclonal antibody to ROCK1 (Rho Kinase) (aa1114-1126) from human. It is reactive with human, mouse, rat and other species. Validated for WB.
- Application
- IHC, WB
- Reactivity
- Bov, Can, Hr, Hu, Mml, Ms, NHP, Rt
- Predicted Reactivity
- Rabbit
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- p160 Rho-associated kinase | p160ROCK | p160 ROCK-1 | p160-ROCK | p160RhoK | Rho-associated kinase | ROCK1 | RockI | Rok | Rok alpha | Rho-alpha kinase | p160 rho-kinase | Rho kinase | ROCK-I | Rokalpha | ROCK-1
- Recommended Dilution: WB
- 1 - 2 µg/ml
- UniProt Number
- Q13464
- NCBI GenBank
- NM_005406 | NP_005397.1
- SwissProt
- Q13464
Target
- Target
- Human Rho Kinase / ROCK1
- Antigen Species
- Human
- Epitope
- This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.
- Gene Name
- ROCK1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 154C1465
- Isotype
- IgG
Immunogen
- Immunogen
- This antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to residues 1114-1126 GNLPESRIEGWLS of human ROCK-1. Immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Bovine, Dog, Bat, Horse, Opossum, Platypus (100%); Rabbit (92%); Chicken (85%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.05% Sodium Azide, 0.2% Gelatin
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
