LSBio
Rabbit Anti-Mouse IgG2b Secondary Antibody (AP)

Dot Blot of Rabbit anti-Mouse IgG2b Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG2b. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Rabbit anti-Mouse IgG2b Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 1 HR at RT.

Rabbit Anti-Mouse IgG2b Secondary Antibody (AP)

Secondary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot
Reactivity:Mouse
Format:Liquid
See all Mouse IgG2b antibodies →
$426.00each
Catalog Number: LS-C60798-1

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Overview

Description
Mouse IgG2b antibody LS-C60798 is an AP-conjugated rabbit polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB.
Application
DB, ELISA, IHC, Other, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:8000 - 1:32000
Recommended Dilution: IHC
1:200 - 1:1000
Recommended Dilution: WB
1:1000 - 1:3000

Target

Target
Mouse IgG2b
Antigen Species
Mouse
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Mouse IgG2b heavy chain.

Format

Conjugate/Label
Alkaline Phosphatase
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.09% Sodium Azide, 50% (v/v) Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C. Do not freeze.
Recommended Storage Buffer
0.05 M Tris-Chloride|0.15 M NaCl

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