LSBio
Polyclonal Goat Anti-Mouse IgG2b Secondary Antibody (AP, IHC, WB)

Dot Blot of Mouse IgG2b Antibody Alkaline Phosphatase Conjugated Pre-absorbed. Antigen: Mouse IgG2b. Load: Lane 1 - 100ng Lane 2 - 33.33ng Lane 3 - 11.11ng Lane 4 - 3.70ng Lane 5 - 1.23ng. Primary antibody: n/a. Secondary antibody: Mouse IgG2b Antibody Alkaline Phosphatase Conjugated Pre-absorbed at 1:1,000 for 60 min at RT.

Polyclonal Goat Anti-Mouse IgG2b Secondary Antibody (AP, IHC, WB)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Mouse
Format:Liquid
See all Mouse IgG2b antibodies →
$393.00each
Catalog Number: LS-C209491-1

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Overview

Description
Mouse IgG2b antibody LS-C209491 is an AP-conjugated goat polyclonal antibody. Validated for DB, ELISA, IHC and WB.
Application
DB, ELISA, IHC, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IHC
1:200 - 1:1000
Recommended Dilution: WB
1:500 - 1:2000

Target

Target
Mouse IgG2b
Antigen Species
Mouse
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, Mouse Serum and Mouse IgG. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Mouse IgG2b heavy chain.

Format

Conjugate/Label
Alkaline Phosphatase
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Do not freeze. Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing will result in a substantial loss of activity.
Recommended Storage Buffer
0.05 M Tris-Chloride|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.