Anti-Human IgA Secondary Antibody
Catalog Number: LS-C758146-1
1 mg / $306.00
Antibody: Human Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Western blot of Rabbit anti-Human Fc antibody. Lane 1: Human Fc. Lane 2: none. Load: 100 ng per lane. Primary antibody: Human Fc antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Human Fc. Other band(s): Human Fc splice variants and isoforms.
Rabbit Anti-Human IgG Fc Secondary Antibody
Secondary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$408.00each
Catalog Number: LS-C60585-5
Catalog Number: LS-C60585
For bulk orders or custom formulations:
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- Description
- Human IgG Fc antibody LS-C60585 is an unconjugated rabbit polyclonal antibody. Validated for ELISA, IE, IHC and WB.
- Application
- ELISA, IHC, Other, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Recommended Dilution: ELISA
- 1:375000
- Recommended Dilution: IHC
- 1:1000 - 1:6000
- Recommended Dilution: WB
- 1:3000 - 1:30000
Target
- Target
- Human IgG Fc
- Antigen Species
- Human
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab).
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Anti-Human IgG F(c) fragment was produced by repeated immunization with Human IgG F(c) fragment in rabbit.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 5.2 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl