LSBio
Mouse Anti-Human IgG Fc Secondary Antibody (clone 3D8.H7.D4)

Western Blot of Mouse anti-Human Fc antibody. Lane 1: Human IgG. Lane 2: Human Fc. Lane 3: Human Fab. Lane 4: Human Fab2. Lane 5: Human IgM. Lane 6: Human IgM Fc5 mu . Load: 50 ng per lane. Primary antibody: Human Fc monoclonal antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Human Fc.

Mouse Anti-Human IgG Fc Secondary Antibody (clone 3D8.H7.D4)

Secondary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Other, Western Blot
Reactivity:Human
Format:Liquid
See all Human IgG Fc antibodies →
$384.00each
Catalog Number: LS-C209487-100

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Overview

Description
Human IgG Fc antibody LS-C209487 is an unconjugated mouse monoclonal antibody. Validated for ELISA, IE and WB.
Application
ELISA, Other, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:1000 - 1:10000
Recommended Dilution: WB
1:1000 - 1:2000

Target

Target
Human IgG Fc
Antigen Species
Human
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG, Human Serum and Human F(c). No reaction was observed against Human F(ab).

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
3D8.H7.D4
Isotype
IgG1
Light Chain
Kappa Light Chain

Immunogen

Immunogen
Anti-Human IgG F(c) monoclonal antibody was produced by repeated immunization with Human IgG F(c) fragment in mice.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.