LSBio
Rabbit Anti-Chicken IgY Secondary Antibody (HRP)

ELISA results of purified F(ab')2 anti-Chicken IgG Peroxidase Conjugated Antibody tested against purified chicken IgG. Each well was coated in duplicate with 1.0 µg of chicken IgG (purple line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. substrate.

Rabbit Anti-Chicken IgY Secondary Antibody (HRP)

Secondary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Chicken
Format:Lyophilized
See all Chicken IgY antibodies →
$393.00each
Catalog Number: LS-C61278-500

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Overview

Description
Chicken IgY antibody LS-C61278 is an HRP-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB. Cited in 1 publication.
Application
DB, ELISA, IHC, WB
Reactivity
Ck
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:7500 - 1:30000
Recommended Dilution: IHC
1:500 - 1:2500
Recommended Dilution: WB
1:1000 - 1:3000

Target

Target
Chicken IgY
Antigen Species
Chicken
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG and Chicken Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Chicken IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl
Immune Milieu Established by Postpartum Liver Involution Promotes Breast Cancer Liver Metastasis. Alexandra Q Bartlett, Nathan D Pennock, Alex Klug, Pepper Schedin. Cancers. 2021 Apr;13:1698. PubMed: 33916683 PMC: PMC8038410

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