LSBio
Donkey Anti-Chicken IgY Secondary Antibody (AP)

Western Blot of Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule Lane 2: Chicken IgG F(c) Fragment Lane 3: Chicken IgG Fab Fragment Lane 4: Chicken IgM Whole Molecule Lane 5: Chicken Serum All samples were reduced. Load: 50 ng per lane.

Donkey Anti-Chicken IgY Secondary Antibody (AP)

Secondary AntibodyLSBio Guarantee
Antibody:Donkey Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot
Reactivity:Chicken
Format:Liquid
See all Chicken IgY antibodies →
$421.00each
Catalog Number: LS-C153875-1

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Overview

Description
Chicken IgY antibody LS-C153875 is an AP-conjugated donkey polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB.
Application
DB, ELISA, IHC, Other, WB
Reactivity
Ck
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IHC
1:200 - 1:1000
Recommended Dilution: WB
1:500 - 1:2500

Target

Target
Chicken IgY
Antigen Species
Chicken
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, anti-Alkaline Phosphatase, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.

Antibody

Host Species
Donkey
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Chicken IgG whole molecule

Format

Conjugate/Label
Alkaline Phosphatase
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.1% Sodium Azide, 50% (v/v) Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C. Do not freeze.
Recommended Storage Buffer
0.05 M Tris-Chloride|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.