Immunofluorescence staining of MCF-7 cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
PVRL4 / Nectin 4 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$300.00each
Catalog Number: LS-C680939-50
Catalog Number: LS-C680939
For bulk orders or custom formulations:
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- Description
- Nectin 4 antibody LS-C680939 is an unconjugated rabbit polyclonal antibody to Nectin 4 (PVRL4) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, IHC-P, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- EDSS1 | Nectin 4 | Ig superfamily receptor LNIR | Poliovirus receptor-like 4 | LNIR | Nectin-4 | Poliovirus receptor-related 4 | PVRL4
- UniProt Number
- Q96NY8
- NCBI GenBank
- NM_030916 | NP_112178.2
- SwissProt
- Q96NY8
Target
- Target
- Human PVRL4 / Nectin 4
- Antigen Species
- Human
- Gene Name
- NECTIN4
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant Human Nectin-4 protein (91-199AA)
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
- Recommended Storage Buffer
- 0.01 M PBS