HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTCH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTCH1.
PTCH1 / Patched 1 Antibody (clone OTI5C7)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$459.00each
Catalog Number: LS-C114391-100
Catalog Number: LS-C114391
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Patched 1 antibody LS-C114391 is an unconjugated mouse monoclonal antibody to human Patched 1 (PTCH1). Validated for IF, IHC and WB. Cited in 1 publication.
- Application
- IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- NBCCS | Patched 1 | Patched homolog 1 | Protein patched homolog 1 | PTC1 | PTCH | PTCH protein +12b | PTCH protein -10 | PTCH protein -3,4,5 | Patched | Patched protein homolog 1 | PTCH1 | BCNS | HPE7 | Patched homolog | PTC | PTCH protein +4 | PTCH11
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC-P
- 1:50
- Recommended Dilution: WB
- 1:500
- UniProt Number
- Q13635
- NCBI GenBank
- NM_000264 | NP_000255.2
- SwissProt
- Q13635
Target
- Target
- Human PTCH1 / Patched 1
- Antigen Species
- Human
- Epitope
- Human PTCH1 / Patched
- Gene Name
- PTCH1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI5C7
- Isotype
- IgG2b
Immunogen
- Immunogen
- Human recombinant protein fragment corresponding to amino acids 122-436 of human PTCH1 (NP_000255) produced in E. coli.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. Yang Gu , Yanying Wang , Luyun He , Jiahang Zhang , Xiaoxiao Zhu , Nian Liu, Jianyi Wang, Tiankun Lu, Lei He, Yong Tian, Zusen Fan. Molecular cancer. 2021 October;20:132. PubMed: 34649567 PMC: PMC8515748