Immunofluorescence staining of HepG2 cells with Prf1 Antibody at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
PRF1 / Perforin Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general) |
| Reactivity: | Human |
| Format: | Liquid |
$300.00each
Catalog Number: LS-C397037-50
Catalog Number: LS-C397037
For bulk orders or custom formulations:
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- Description
- Perforin antibody LS-C397037 is an unconjugated rabbit polyclonal antibody to human Perforin (PRF1). Validated for ELISA.
- Application
- ELISA, IHC
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HPLH2 | Perforin | PRF1 | p1 | Perforin1 | PFP | Cytolysin | FLH2 | Perforin-1
- UniProt Number
- P14222
- NCBI GenBank
- NM_005041 | NP_005032.2
- SwissProt
- P14222
Target
- Target
- Human PRF1 / Perforin
- Antigen Species
- Human
- Epitope
- Human PRF1 / Perforin
- Gene Name
- PRF1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant human Perforin-1 protein.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS