LSBio
Polyclonal Rabbit Anti-Pig IgG Secondary Antibody (AP, IHC, WB)

Western Blot of Alkaline Phosphatase conjugated Rabbit anti-Swine IgG antibody. Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase swine secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Rabbit Anti-Pig IgG Secondary Antibody (AP, IHC, WB)

Secondary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Pig
Format:Liquid
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$368.00each
Catalog Number: LS-C61171-1

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Overview

Description
Pig IgG antibody LS-C61171 is an AP-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Application
DB, ELISA, IHC, WB
Reactivity
Por
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Alternative Names
Porcine IgG | Swine IgG
Recommended Dilution: ELISA
1:14000
Recommended Dilution: IHC
1:200 - 1:1000
Recommended Dilution: WB
1:500 - 1:2500

Target

Target
Pig IgG
Antigen Species
Pig
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Swine IgG, and Swine Serum.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Swine IgG whole molecule.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Alkaline Phosphatase
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C. Do not freeze.
Recommended Storage Buffer
0.05 M Tris-Chloride|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.