LSBio
Polyclonal Rabbit anti-Mouse IL27 Antibody (Biotin, IHC, WB)

Western Blot of Biotin Conjugated Rabbit anti-IL-27/p28 antibody. Lane 1: Mouse IL-27/p28. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Biotin rabbit secondary antibody at 1:1,000 for 60 min at RT.

Polyclonal Rabbit anti-Mouse IL27 Antibody (Biotin, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Mouse
Format:Lyophilized
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$564.00each
Catalog Number: LS-C154146-100

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Overview

Description
IL27 antibody LS-C154146 is a biotin-conjugated rabbit polyclonal antibody to mouse IL27. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Ms
Predicted Reactivity
Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
IL-27 | IL-27 p28 subunit | IL30 | Interleukin 27 | Interleukin-27 subunit alpha | IL27A | Interleukin 30 | IL-27-A | IL27 | IL27p28 | IL-27 subunit alpha | IL-27A | IL27-A | p28
Recommended Dilution: ELISA
1:20000 - 1:100000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000
UniProt Number
Q8NEV9
NCBI GenBank
NM_145659 | NP_663634.2
SwissProt
Q8NEV9

Target

Target
Mouse IL27
Antigen Species
Mouse
Epitope
This antibody is specific for mouse IL-27/p28 protein. A BLAST analysis was used to suggest cross-reactivity with IL-27A/p28 from mouse sources based on 100% homology with the immunizing sequence. Based on 90% or greater positive homology, there is a chance of cross-reactivity to rat. Cross-reactivity with IL-27 from other sources has not been determined.
Gene Name
IL27

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL27/p28 protein.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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