LSBio
IHC-plus™ IL27 Antibody

Anti-IL-27 antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ IL27 Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse
Format:Liquid
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$470.00each
Catalog Number: LS-B2719-0.05

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Overview

Description
IL27 antibody LS-B2719 is an unconjugated rabbit polyclonal antibody to IL27 from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
IL-27 | IL-27 p28 subunit | IL30 | Interleukin 27 | Interleukin-27 subunit alpha | IL27A | Interleukin 30 | IL-27-A | IL27 | IL27p28 | IL-27 subunit alpha | IL-27A | IL27-A | p28
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
2 - 4 µg/ml
UniProt Number
Q8NEV9
SwissProt
Q8NEV9

Target

Target
IL27
Antigen Species
Human
Gene Name
IL27

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
A 14 AA synthetic peptide near the center of human IL-27. The immunogen is located within aa 80-130 of IL-27.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS containing 0.02% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. Morandi F, Di Carlo E, Ferrone S, Petretto A, Pistoia V, Airoldi I. Journal of immunology (Baltimore, Md. : 1950). 2014 Mar;192:2634-42.

PubMed: 24554774


IL-27 induces the expression of IDO and PD-L1 in human cancer cells. Carbotti G, Barisione G, Airoldi I, Mezzanzanica D, Bagnoli M, Ferrero S, Petretto A, Fabbi M, Ferrini S. Oncotarget. 2015;6:43267-80.

PubMed: 26657115 · PMC: PMC4791231

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